Diffusion constant for the repton model of gel electrophoresis
نویسندگان
چکیده
The repton model is a simple model of the “reptation” motion by which DNA diffuses through a gel during electrophoresis. In this paper we show that the model can be mapped onto a system consisting of two types of particles with hard-sphere interactions diffusing on a one-dimensional lattice. Using this mapping we formulate an efficient Monte Carlo algorithm for the model which allows us to simulate systems more than twice the size of those studied before. Our results confirm scaling hypotheses which have previously been put forward for the model. We also show how the particle version of the model can be used to construct a transfer matrix which allows us to solve exactly for the diffusion constant of small repton systems. We give results for systems of up to 20 reptons. Typeset using REVTEX
منابع مشابه
Gel Electrophoresis of Dna — New Measurements and the Repton Model at High Fields
New experimental data are presented on the gel electrophoresis of DNA. Experiment was made for molecules of length 173 kbp, in 1 percent agarose gel, in TAE 1× buffer and the field intensity between 5 and 9V/cm. The results are compared with our computer simulations, performed within the repton model of Duke and Rubinstein. The ranges of field and molecule length are determined, where the geome...
متن کاملDiiusion Constant for the Repton Model of Gel Electrophoresis
The repton model is a simple model of the \reptation" motion by which DNA diiuses through a gel during electrophoresis. In this paper we show that the model can be mapped onto a system consisting of two types of particles with hard-sphere interactions diiusing on a one-dimensional lattice. Using this mapping we formulate an eecient Monte Carlo algorithm for the model which allows us to simulate...
متن کاملExact curvilinear diffusion coefficients in the repton model.
The Rubinstein-Duke or repton model is one of the simplest lattice model of reptation for the diffusion of a polymer in a gel or a melt. Recently, a slightly modified model with hardcore interactions between the reptons has been introduced. The curvilinear diffusion coefficients of both models are exactly determined for all chain lengths. The case of periodic boundary conditions is also conside...
متن کاملA Novel Spot-Enhancement Anisotropic Diffusion Method for the Improvement of Segmentation in Two-dimensional Gel Electrophoresis Images, Based on the Watershed Transform Algorithm
Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks, artifacts and noise. Watershed transform is one of the common methods for image segmentation. Nevertheless, in 2DGE image segmentation, the no...
متن کاملMolecular Typing of Acinetobacter Baumannii Clinical Strains in Tehran by Pulsed-Field Gel Electrophoresis
Background & Objective : Currently, Acinetobacter baumannii is an important nosocomial pathogen insofar as its hospital outbreaks have been described from various geographical areas. Since the discrimination of strains within a species is important for delineating nosocomial outbreaks, this study was conducted with the aim of genotyping the A. baumannii clinical strains in Tehran via the pulsed...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 1997